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Pancreatic Cancer

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1882)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xii
  2. Subtyping of IPMN

    • Toru Furukawa
    Pages 1-8
  3. Pancreatic Microtumors: A Novel 3D Ex Vivo Testing Platform

    • Mackenzie Lee Goodwin, Sumi Kanthraj Urs, Diane M. Simeone
    Pages 73-80
  4. Osteoclast Differentiation Assay

    • Jingxuan Yang, Xiaohong Bi, Min Li
    Pages 143-148
  5. Primary Cultures for Pancreatic Stellate Cells (PSCs)

    • Lei Tian, Zipeng Lu, Yi Miao
    Pages 149-155
  6. Cytokine Profiling and Orthotopic Xenografing of Pancreatic Stellate Cells

    • Dong Qian, Lei Tian, Zipeng Lu, Yi Miao
    Pages 157-160
  7. Quantitative Method to Track Proteolytic Invasion in 3D Collagen

    • Kazumi Ebine, Christina R. Chow, Hidayatullah G. Munshi
    Pages 161-169
  8. Detection and Quantification of Macropinosomes in Pancreatic Tumors

    • Szu-Wei Lee, Basheer Alas, Cosimo Commisso
    Pages 171-181
  9. Evaluating Stress Granules in Pancreatic Cancer In Vitro and In Vivo

    • Edward Sim, Elena Irollo, Elda Grabocka
    Pages 183-195
  10. Methods for Monitoring Macroautophagy in Pancreatic Cancer Cells

    • Chiara Vidoni, Alessandra Ferraresi, Christian Seca, Eleonora Secomandi, Ciro Isidoro
    Pages 197-206
  11. Measurement of Reactive Oxygen Species by Fluorescent Probes in Pancreatic Cancer Cells

    • Yongde Luo, Dan Wang, James L. Abbruzzese, Weiqin Lu
    Pages 207-219

About this book

This third edition volume expands on the previous editions with discussions on the latest developments in 2D- and 3D-organoid cultures that allow for the execution of precision medicine, and in vitro studies of tumor cell growth and invasion. The chapters in this book explore protocols for evaluating macropinocytosis, stress granules, autophagy, metabolic alterations, and reactive oxygen species, which have proven to be possible therapeutic opportunities. The protocols described in this book look at both human and animal models, ranging from in vitro and in vivo, and will aid in future research of pancreatic cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and comprehensive, Pancreatic Cancer: Methods and Protocols, Third Edition is a valuable resource for scientists and researchers who are interested in learning more about ways of improving patient care for pancreatic cancer.

Editors and Affiliations

  • Department of Pathology and Cell Biology, Columbia University Medical Center, New York, USA

    Gloria H. Su

About the editor

   

Bibliographic Information

  • Book Title: Pancreatic Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Gloria H. Su

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8879-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8878-5Published: 31 October 2018

  • Softcover ISBN: 978-1-4939-9406-9Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8879-2Published: 30 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 337

  • Number of Illustrations: 11 b/w illustrations, 40 illustrations in colour

  • Topics: Cancer Research

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access