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T-Cell Motility

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Navin Kumar Verma

    1. Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1930)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility

    • Navin Kumar Verma, Dermot Kelleher
    Pages 1-9

  3. Isolation of Human Peripheral Blood T-Lymphocytes

    • Atish Kizhakeyil, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Navin Kumar Verma
    Pages 11-17

  4. A Laboratory Model to Study T-Cell Motility

    • Navin Kumar Verma, Shyam Mohan Palapetta, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan et al.
    Pages 19-23

  5. Quantification of T-Cell Migratory Phenotypes Using High-Content Analysis

    • Aik Seng Ng, Seow Theng Ong, Dermot Kelleher, Navin Kumar Verma
    Pages 25-32

  6. Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time

    • Seow Theng Ong, Navin Kumar Verma
    Pages 33-40

  7. Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signaling Cross-Talks in Motile T-Cells

    • Seow Theng Ong, Graham D. Wright, Navin Kumar Verma
    Pages 41-50

  8. Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis

    • Praseetha Prasannan, Navin Kumar Verma
    Pages 51-57

  9. Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes

    • Atish Kizhakeyil, Navin Kumar Verma
    Pages 59-66

  10. GapmeR-Mediated Gene Silencing in Motile T-Cells

    • Mobashar Hussain Urf Turabe Fazil, Seow Theng Ong, Madhavi Latha Somaraju Chalasani, Atish Kizhakeyil, Navin Kumar Verma
    Pages 67-73

  11. Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices Using Confocal Microscopy

    • Emmanuel Donnadieu, Yvonne Michel, Martin-Leo Hansmann
    Pages 75-82

  12. Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes

    • Praseetha Prasannan, Rathi Saravanan, Navin Kumar Verma
    Pages 83-90

  13. Immunometabolomic Phenotyping of Motile T-Cells

    • Madhavi Latha Somaraju Chalasani, Gaelin Looi Wen Dong, Navin Kumar Verma
    Pages 91-98

  14. Profiling Activity of Cellular Kinases in Migrating T-Cells

    • Chandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy et al.
    Pages 99-113

  15. Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes

    • Malin Samuelsson, Lena M. Svensson
    Pages 115-122

  16. A Protocol to Study T-Cell Signaling in an Immune Synapse by Microscopy

    • Mobashar Hussain Urf Turabe Fazil, Pankaj Kumar, Navin Kumar Verma
    Pages 123-128

  17. Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides

    • Madhavi Latha Somaraju Chalasani, Rajamani Lakshminarayanan, Navin Kumar Verma
    Pages 129-138

  18. Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility

    • Geoffrey W. Payne, Kevin Mitchell, Stephanie L. Sellers
    Pages 139-147

  19. Computational Analysis of Protein–Protein Interactions in Motile T-Cells

    • Sunil Kumar, Mobashar Hussain Urf Turabe Fazil, Khurshid Ahmad, Minaketan Tripathy, Jagath Chandana Rajapakse, Navin Kumar Verma
    Pages 149-156

  20. Back Matter

    Pages 157-158
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Keywords

About this book

This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields ofimmunology, T-cell biology, biochemistry, molecular biology, and imaging.



Editors and Affiliations

  • Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore

    Navin Kumar Verma

Bibliographic Information

  • Book Title: T-Cell Motility

  • Book Subtitle: Methods and Protocols

  • Editors: Navin Kumar Verma

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9036-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9035-1Published: 05 January 2019

  • eBook ISBN: 978-1-4939-9036-8Published: 04 January 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 158

  • Number of Illustrations: 3 b/w illustrations, 28 illustrations in colour

  • Topics: Immunology, Cell Biology

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