Abstract
Daily alterations of routines and protocols create high, yet so far unmet demands for intelligent reaction monitoring, quality control and data management in molecular biology laboratories. To meet such needs, the “internet of things” is implemented here. We propose an approach which combines direct tracking of lab tubes, reactions and racks with a comprehensive data management system. Reagent tubes in this system are tagged with 2D data matrices or imprinted RFID-chips using a unique identification number. For each tube, individual content and all relevant information based on conducted experimental procedures are stored in an experimental data management system. This information is managed automatically but allow scientists to engage and interfere via user-friendly graphical interface. Tagged tubes are used in connection with a detectable RFID-tagged rack. We show that reaction protocols, HTS storage and complex reactions are easily planned and controlled.
Keywords
Authors (Arthur Neuberger and Zeeshan Ahmed) contribute equally to the work.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Huang, G.Q., Zhang, Y.F., Jiang, P.Y.: RFID-based wireless manufacturing for walking-worker assembly islands with fixed-position layouts. Robot Comput.-Integr. Manuf. 23, 469–477 (2007)
Atzori, L., Iera, A., Morabito, G.: The internet of things: a survey. Comput. Netw. 54, 2787–2805 (2010)
Spolaczyk, R: Apparatus for handling liquids and a process for operating the device (2004). https://www.google.com/patents/US6819437
Becher, H., Renz, M.: Pipetting robot for laboratory use (2013). https://www.google.com/patents/USD674110
Mann, K.H., Lang, A., Nowak, E., Sattler, S., Schels, H.D.: Verfahren zur Analyse von Probenflüssigkeiten (1995). https://encrypted.google.com/patents/EP0637750A2?cl=it
Davis, R., Geiger, B., Gutierrez, A., Heaser, J., Veeramani, D.: Tracking blood products in blood centres using radio frequency identification: a comprehensive assessment. Vox Sang. 97, 50–60 (2009)
Hohberger, C., Davis, R., Briggs, L., Gutierrez, A., Veeramani, D.: Applying radio-frequency identification (RFID) technology in transfusion medicine. Biol.: J. Int. Assoc. Biol. Stand. 40, 209–213 (2012)
Neuberger, A., Ahmed, Z., Dandekar, T.: A system for non-contact monitoring of reaction vessels with electronic bearing support, preparation of suitable vessels and monitoring technology (2014). https://www.google.com/patents/DE102014005549A1?cl=en
Mobley, A., Linder, S.K., Braeuer, R., Ellis, L.M., Zwelling, L.: A survey on data reproducibility in cancer research provides insights into our limited ability to translate findings from the laboratory to the clinic. PLoS ONE 8, e63221 (2013)
Linshiz, G., Stawski, N., Poust, S., Bi, C., Keasling, J.D., Hillson, N.J.: PaR-PaR laboratory automation platform. ACS Synth. Biol. 2, 216–222 (2013)
Ananthanarayanan, V., Thies, W.: Biocoder: a programming language for standardizing and automating biology protocols. J. Biol. Eng. 4, 13 (2010)
Soldatova, L.N., Aubrey, W., King, R.D., Clare, A.: The EXACT description of biomedical protocols. Bioinformatics 24, i295–i303 (2008)
Ellefson, L.P.: Label stripping apparatus for label printers (1977). http://www.google.tl/patents/US4036132
Excoffier, J.L., Ehrlich, L.E.: Sample identification utilizing RFID tags (2007). http://www.google.tl/patents/US20060213964
Trueeb, H., Birrer, A., Brauner, T.: Method and system to localise and identify test tubes (2010). https://www.google.com/patents/US20100025464
Trueeb, H., Birrer, A., Brauner, T.: Laboratory device for processing samples and methods using the same (2012). http://www.google.tl/patents/US8197750
Pedrazzini, G.: System for automatically identifying, conveying and addressing biological material specimens (2010). https://www.google.com/patents/US20100300831
Ullmann, A., Böhm, M.: Rfid transponder (2010). https://www.google.com/patents/US20100243742
Barrett, T.J., Check, C.J., Mauch, C.A., Lauria, M., Scharpf, P.G.: RFID label printing system (2003). https://www.google.ms/patents/US6593853
Hammond, P.A., Ali, D., Cumming, D.R.: A system-on-chip digital pH meter for use in a wireless diagnostic capsule. IEEE Trans. Biomed. Eng. 52, 687–694 (2005)
Johannessen, E.A., Wang, L., Cui, L., Tang, T.B., Ahmadian, M., Astaras, A., Reid, S.W., Yam, P.S., Murray, A.F., Flynn, B.W., Beaumont, S.P., Cumming, D.R., Cooper, J.M.: Implementation of multichannel sensors for remote biomedical measurements in a microsystems format. IEEE Trans. Bio-Med. Eng. 51, 525–535 (2004)
King, R.D., Whelan, K.E., Jones, F.M., Reiser, P.G., Bryant, C.H., Muggleton, S.H., Kell, D.B., Oliver, S.G.: Functional genomic hypothesis generation and experimentation by a robot scientist. Nature 427, 247–252 (2004)
Soldatova, L.N., Clare, A., Sparkes, A., King, R.D.: An ontology for a robot scientist. Bioinformatics 22, e464–e471 (2006)
Kitson, P.J., Rosnes, M.H., Sans, V., Dragone, V., Cronin, L.: Configurable 3D-Printed millifluidic and microfluidic ‘lab on a chip’ reactionware devices. Lab Chip 12, 3267–3271 (2012)
Wu, G., Bashir-Bello, N., Freeland, S.J.: The synthetic gene designer: a flexible web platform to explore sequence manipulation for heterologous expression. Protein Expr. Purif. 47, 441–445 (2006)
Vehniaeinen, A., Gustafsson, H., Koskinen, T.: Method and a system for producing nanocellulose, and nanocellulose (2011). https://www.google.ch/patents/US20130303749
Das, R., Kiley, P.J., Segal, M., Norville, J., Yu, A.A., Wang, L., Trammell, S.A., Reddick, L.E., Kumar, R., Stellacci, F., Lebedev, N., Schnur, J., Bruce, B.D., Zhang, S., Baldo, M.: Integration of photosynthetic protein molecular complexes in solid-state electronic devices. Nano Lett. 4, 1079–1083 (2004)
Acknowledgements
We thank BMBF for support (grant number 031L0129B).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Nature Switzerland AG
About this paper
Cite this paper
Neuberger, A., Ahmed, Z., Dandekar, T. (2020). IntelliEppi: Intelligent Reaction Monitoring and Holistic Data Management System for the Molecular Biology Lab. In: Arai, K., Bhatia, R. (eds) Advances in Information and Communication. FICC 2019. Lecture Notes in Networks and Systems, vol 69. Springer, Cham. https://doi.org/10.1007/978-3-030-12388-8_28
Download citation
DOI: https://doi.org/10.1007/978-3-030-12388-8_28
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-12387-1
Online ISBN: 978-3-030-12388-8
eBook Packages: Intelligent Technologies and RoboticsIntelligent Technologies and Robotics (R0)