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Method for Living Cell Mechanical Properties Evaluation from Force-Indentation Curves

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Automation 2019 (AUTOMATION 2019)

Part of the book series: Advances in Intelligent Systems and Computing ((AISC,volume 920))

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Abstract

Living cells mechanical properties establishment from Atomic force microscopy (AFM) force-separation curves is a challenge because the calculated Young’s modulus depends on the applied mathematical model. The more reliable results can be obtained using finite element models. In this work, yeast cells with different mechanical properties were measured by AFM. To change cells mechanical properties, yeasts were immersed in 9, 10-phenanthrenequinone, which changed cells’ membranes elasticity. 3D finite element model of the whole cell was created to calculate reacting force when AFM tip indents the cell in the same way as in the real experiment. It was found that our model is capable to draw the information about cells mechanical properties and visco-elastic behavior of cells membranes.

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Acknowledgement

This research was funded by the European Social Fund according to the activity “Development of Competences of Scientists, other Researchers and Students through Practical Research Activities” of Measure No. 09.3.3-LMT-K-712. Project No. 09.3.3-LMT-K-712-02-0137.

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Authors and Affiliations

  1. Vilnius Gediminas Technical University, J. Basanaviciaus 28, 03224, Vilnius, Lithuania

    Inga Morkvenaite-Vilkonciene, Raimundas Vilkoncius, Juste Rozene, Andrius Dzedzickis & Vytautas Bučinskas

  2. Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania

    Inga Morkvenaite-Vilkonciene, Antanas Zinovicius, Almira Ramanaviciene & Arunas Ramanavicius

  3. Center for Physical Sciences and Technology, Sauletekio 3, Vilnius, Lithuania

    Oleksii Balitskyi

Authors
  1. Inga Morkvenaite-Vilkonciene
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  2. Raimundas Vilkoncius
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  3. Juste Rozene
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  4. Antanas Zinovicius
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  5. Oleksii Balitskyi
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  6. Almira Ramanaviciene
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  7. Arunas Ramanavicius
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  8. Andrius Dzedzickis
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  9. Vytautas Bučinskas
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Corresponding author

Correspondence to Inga Morkvenaite-Vilkonciene .

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Editors and Affiliations

  1. Industrial Research Institute for Automation and Measurements PIAP Al, Warsaw, Poland

    Roman Szewczyk

  2. Industrial Research Institute for Automation and Measurements PIAP Al, Warsaw, Poland

    Cezary Zieliński

  3. Industrial Research Institute for Automation and Measurements PIAP Al, Warsaw, Poland

    Małgorzata Kaliczyńska

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Morkvenaite-Vilkonciene, I. et al. (2020). Method for Living Cell Mechanical Properties Evaluation from Force-Indentation Curves. In: Szewczyk, R., Zieliński, C., Kaliczyńska, M. (eds) Automation 2019. AUTOMATION 2019. Advances in Intelligent Systems and Computing, vol 920. Springer, Cham. https://doi.org/10.1007/978-3-030-13273-6_61

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