Abstract
Constituents of microalgae and sample preparation for UPLC-ELSD and GC-MS analyses are described. Bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters are derivatized by using transesterification with sodium methoxide to form fatty acid methyl esters. Compounds containing free hydroxyl groups, either present originally or formed during previous step, like free fatty acids, sterols, α-tocopherol, phytol, and nonesterified alkoxyglycerols, are trimethylsilylated. The compounds in algal lipid extract are subsequently derivatized by these two steps.
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Acknowledgment
This project was supported by the EU-funded SEABIOTECH project (FP7-SBT-KBBE-311932, http://www.seabiotech.org/). The authors thank Dr. Marilyn Wiebe who was kindly supplying the Euglena gracilis biomass. The authors owe thanks to Airi Hyrkäs for excellent technical assistance in sample preparation and optimization of the running conditions in UPLC-ELSD analyses, especially.
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Seppänen-Laakso, T., Nygren, H., Rischer, H. (2017). UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods. In: Spilling, K. (eds) Biofuels from Algae. Methods in Molecular Biology, vol 1980. Humana, New York, NY. https://doi.org/10.1007/7651_2017_109
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DOI: https://doi.org/10.1007/7651_2017_109
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