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Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells

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Stem Cells and Aging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2045))

Abstract

Primordial germ cells (PGCs), the precursors of gametes, are the only cells capable of acquiring totipotency upon fertilization, but the molecular mechanisms regulating germ cell characteristics have not been fully elucidated. Although intracellular metabolic status and regulation are responsible for the control of cell function and differentiation, little is known about the metabolic features of PGCs. Here, we describe use of an integrated metabolomic, proteomic, and energy metabolic analysis method to comprehensively elucidate the metabolic characteristics of PGCs using mass spectrometry.

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Acknowledgments

Y.M. was supported by a Grant-in-Aid for Scientific Research (KAKENHI) in the Innovative Areas, “Mechanisms regulating gamete formation in animals” (grant #25114003) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by AMED-CREST (grant #JP17gm0510017h) from the Japan Agency for Medical Research and Development.

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Authors and Affiliations

  1. Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer (IDAC), Tohoku University, Sendai, Miyagi, Japan

    Yohei Hayashi & Yasuhisa Matsui

  2. Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan

    Yohei Hayashi & Yasuhisa Matsui

  3. The Japan Agency for Medical Research and Development-Core Research for Evolutional Science and Technology (AMED-CREST), Tokyo, Japan

    Yohei Hayashi & Yasuhisa Matsui

  4. Center for Regulatory Epigenome and Diseases, Tohoku University School of Medicine, Sendai, Miyagi, Japan

    Yasuhisa Matsui

Authors
  1. Yohei Hayashi
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  2. Yasuhisa Matsui
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Corresponding author

Correspondence to Yasuhisa Matsui .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Hayashi, Y., Matsui, Y. (2018). Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells. In: Turksen, K. (eds) Stem Cells and Aging . Methods in Molecular Biology, vol 2045. Humana, New York, NY. https://doi.org/10.1007/7651_2018_164

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  • DOI: https://doi.org/10.1007/7651_2018_164

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9712-1

  • Online ISBN: 978-1-4939-9713-8

  • eBook Packages: Springer Protocols

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