Abstract
It has been described that adult tissues contain mesenchymal stem cell populations. The specific areas where stem cells reside are known as niches. Crosstalk between cells and their niche is essential to maintain the correct functionality of stem cell. MSCs present a set of abilities such as migration, invasion, and angiogenic potentials, which make them ideal candidates for cell-based therapies. In order to test the regenerative capacity of these cells, we have described a methodology for the collection and for the evaluation of these mesenchymal precursors from different niches.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bernal A, Fernandez M, Perez LM, San Martin N, Galvez BG (2012) Method for obtaining committed adult mesenchymal precursors from skin and lung tissue. PLoS One 7(12):e53215. https://doi.org/10.1371/journal.pone.0053215
Galvez BG, Sampaolesi M, Barbuti A, Crespi A, Covarello D, Brunelli S, Dellavalle A, Crippa S, Balconi G, Cuccovillo I, Molla F, Staszewsky L, Latini R, Difrancesco D, Cossu G (2008) Cardiac mesoangioblasts are committed, self-renewable progenitors, associated with small vessels of juvenile mouse ventricle. Cell Death Differ 15(9):1417–1428. https://doi.org/10.1038/cdd.2008.75
Galvez BG, San Martin N, Rodriguez C (2009) TNF-alpha is required for the attraction of mesenchymal precursors to white adipose tissue in Ob/ob mice. PLoS One 4(2):e4444. https://doi.org/10.1371/journal.pone.0004444
Hass R, Kasper C, Bohm S, Jacobs R (2011) Different populations and sources of human mesenchymal stem cells (MSC): a comparison of adult and neonatal tissue-derived MSC. Cell Commun Signal 9:12. https://doi.org/10.1186/1478-811X-9-12
De Becker A, Riet IV (2016) Homing and migration of mesenchymal stromal cells: how to improve the efficacy of cell therapy? World J Stem Cells 8(3):73–87. https://doi.org/10.4252/wjsc.v8.i3.73
Weidenhamer NK, Moore DL, Lobo FL, Klair NT, Tranquillo RT (2015) Influence of culture conditions and extracellular matrix alignment on human mesenchymal stem cells invasion into decellularized engineered tissues. J Tissue Eng Regen Med 9(5):605–618. https://doi.org/10.1002/term.1974
Tao H, Han Z, Han ZC, Li Z (2016) Proangiogenic features of mesenchymal stem cells and their therapeutic applications. Stem Cells Int 2016:1314709. https://doi.org/10.1155/2016/1314709
Acknowledgments
This study was supported by the grant from the Spanish Ministry of Science and Innovation (SAF 2015–67911-R) to BGG. BdL is supported by FPU fellowships from the Spanish Ministry of Science and Innovation.
BdL and LMP wrote the manuscript. BGG revised the manuscript. All authors read and approved the final manuscript. We would like to thank all past and present members of the “BGG group”, who have contributed to the design and evolution of these protocols over several years.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media New York
About this protocol
Cite this protocol
de Lucas, B., Pérez, L.M., Gálvez, B.G. (2018). Functional Assays of Stem Cell Properties Derived from Different Niches. In: Turksen, K. (eds) Stem Cell Niche. Methods in Molecular Biology, vol 2002. Humana, New York, NY. https://doi.org/10.1007/7651_2018_175
Download citation
DOI: https://doi.org/10.1007/7651_2018_175
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9507-3
Online ISBN: 978-1-4939-9508-0
eBook Packages: Springer Protocols