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Identification and Characterization of Stem Cells in Oral Cancer

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Stem Cell Niche

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2002))

Abstract

Cancer stem cells (CSCs) are a subpopulation of cells within a heterogeneous tumor that have enhanced biologic properties such as increased capacity for self-renewal, increased tumorigenicity, enhanced differentiation capacity, and resistance to chemo- and radiotherapies. This unit describes protocols to isolate and characterize potential cancer stem cells from a solid tumor (oral cancer). This involves creating a single-cell suspension from tumor tissue, tagging the cell subpopulation of interest, and sorting cells into different populations. Finally, the sorted subpopulations can be evaluated for their ability to meet the functional requirements of a CSC, which primarily include increased tumorigenicity in an in vivo xenograft assay. Mastering the protocols in this unit will allow the researcher to study populations of cells that may have properties of CSCs.

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References

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Acknowledgments

Research support was partly provided by Board of Research in Nuclear Sciences (BRNS) [Number: 37(1)/14/38/2016-BRNS/37276], Department of Atomic Energy (DAE), and Science and Engineering Research Board (SERB) [Number: EMR/2016/001246]. Research infrastructure was partly provided by Fund for Improvement of S&T Infrastructure in Universities and Higher Educational Institutions (FIST) [Number: SR/FST/LSI-025/2014], Department of Science and Technology, Government of India.

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Authors and Affiliations

  1. Department of Life Science, National Institute of Technology Rourkela, Rourkela, Odisha, India

    Sujit K. Bhutia, Prajna P. Naik, Prakash P. Praharaj, Debasna P. Panigrahi, Chandra S. Bhol, Kewal K. Mahapatra, Sarbari Saha & Srimanta Patra

  2. PG Department of Zoology, Vikram Deb (Auto) College, Jeypore, Odisha, India

    Prajna P. Naik

Authors
  1. Sujit K. Bhutia
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  2. Prajna P. Naik
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  3. Prakash P. Praharaj
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  4. Debasna P. Panigrahi
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  5. Chandra S. Bhol
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  6. Kewal K. Mahapatra
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  7. Sarbari Saha
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  8. Srimanta Patra
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Corresponding author

Correspondence to Sujit K. Bhutia .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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Bhutia, S.K. et al. (2018). Identification and Characterization of Stem Cells in Oral Cancer. In: Turksen, K. (eds) Stem Cell Niche. Methods in Molecular Biology, vol 2002. Humana, New York, NY. https://doi.org/10.1007/7651_2018_184

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  • DOI: https://doi.org/10.1007/7651_2018_184

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9507-3

  • Online ISBN: 978-1-4939-9508-0

  • eBook Packages: Springer Protocols

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