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Reconstruction of Regenerative Stem Cell Niche by Cell Aggregate Engineering

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Stem Cell Niche

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2002))

Abstract

The niche plays critical roles in regulating functionality and determining regenerative outcomes of stem cells, for which establishment of favorable microenvironments is in demand in translational medicine. In recent years, the cell aggregate technology has shown immense potential to reconstruct a beneficial topical niche for stem cell-mediated regeneration, which has been recognized as a promising concept for high-density stem cell delivery with preservation of the self-produced, tissue-specific extracellular matrix microenvironments. Here, we describe the basic methodology of stem cell aggregate-based niche engineering and quality check indexes prior to application.

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Acknowledgments

This work was supported by grants from the National Key Research and Development Program of China (2016YFC1102900 and 2016YFC1101400), the General Program of National Natural Science Foundation of China (81570937 and 81470710), and the State Scholarship Fund of China (201603170205).

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Author notes

  1. Bing-Dong Sui and Bin Zhu contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Military Stomatology, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi’an, Shaanxi, China

    Bing-Dong Sui, Bin Zhu, Cheng-Hu Hu, Pan Zhao & Yan Jin

  2. Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi’an, Shaanxi, China

    Bing-Dong Sui, Bin Zhu, Cheng-Hu Hu, Pan Zhao & Yan Jin

  3. Xi’an Institute of Tissue Engineering and Regenerative Medicine, Xi’an, Shaanxi, China

    Cheng-Hu Hu & Yan Jin

Authors
  1. Bing-Dong Sui
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  2. Bin Zhu
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  3. Cheng-Hu Hu
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  4. Pan Zhao
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  5. Yan Jin
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Corresponding author

Correspondence to Yan Jin .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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Sui, BD., Zhu, B., Hu, CH., Zhao, P., Jin, Y. (2018). Reconstruction of Regenerative Stem Cell Niche by Cell Aggregate Engineering. In: Turksen, K. (eds) Stem Cell Niche. Methods in Molecular Biology, vol 2002. Humana, New York, NY. https://doi.org/10.1007/7651_2018_186

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  • DOI: https://doi.org/10.1007/7651_2018_186

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9507-3

  • Online ISBN: 978-1-4939-9508-0

  • eBook Packages: Springer Protocols

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