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Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells

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Stem Cells and Aging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2045))

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Abstract

Multipotent mesenchymal stem cells (MSCs) are an attractive candidate for regeneration of damaged cells, tissues, and organs. Due to limited availabilities, MSC populations must be rapidly expanded to satisfy clinical needs. However, senescence attributed to extensive in vitro expansion compromises the regenerative and therapeutic potential of MSCs. In this chapter, we describe a step-by-step protocol that aims to induce adipogenic and osteogenic differentiation of in vitro aged human MSCs and highlight noteworthy issues that may arise during the process.

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Abbreviations

APC:

Allophycocyanin

BSA:

Bovine serum albumin

ddH2O:

Ultrapure water

DMSO:

Dimethyl sulfoxide

EDTA:

Ethylenediaminetetraacetic acid

FBS:

Fetal bovine serum

FGF-2:

Basic fibroblast growth factor

FITC:

Fluorescein isothiocyanate

HBSS:

Hank’s balanced salt solution

HEPES:

4-(2-Hydroxylethyl)-1-piperazineethanesulfonic acid

hgDMEM:

High glucose Dulbecco’s modified Eagle’s medium

lgDMEM:

Low glucose Dulbecco’s modified Eagle’s medium

MSCs:

Mesenchymal stem cells

PBS:

Phosphate-buffered saline

Pen/Strep:

Penicillin/streptomycin

PerCP-Cy5.5:

Peridinin-chlorophyll protein complex-cyanine 5.5

RPE:

R-phycoerythrin

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Acknowledgments

This work was supported by the City College of New York Research Award (to GAB) and partially by the Professional Staff Congress – City University of New York Research Award (Grant number 61584-00 49 to YHKY).

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Authors and Affiliations

  1. Department of Biomedical Engineering, Grove School of Engineering, The City University of New York – The City College, New York, NY, USA

    Courtney R. Ogando, Gilda A. Barabino & Yueh-Hsun Kevin Yang

Authors
  1. Courtney R. Ogando
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  2. Gilda A. Barabino
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  3. Yueh-Hsun Kevin Yang
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Corresponding author

Correspondence to Yueh-Hsun Kevin Yang .

Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Ogando, C.R., Barabino, G.A., Yang, YH.K. (2018). Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells. In: Turksen, K. (eds) Stem Cells and Aging . Methods in Molecular Biology, vol 2045. Humana, New York, NY. https://doi.org/10.1007/7651_2018_197

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  • DOI: https://doi.org/10.1007/7651_2018_197

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9712-1

  • Online ISBN: 978-1-4939-9713-8

  • eBook Packages: Springer Protocols

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