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Complete Assessment of Multilineage Differentiation Potential of Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cells

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Stem Cells and Aging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2045))

Abstract

The minimal criteria for mesenchymal stem/stromal cell (MSC) identification set by the International Society for Cellular Therapy include plastic adherence, presence and absence of a set of surface antigens and in vitro multilineage differentiation. This differentiation is assessed through stimulation of MSCs with defined combination and concentration of growth factors towards specific lineages and histological confirmation of the presence of differentiated cells. Here we provide protocols for multilineage differentiation, namely, osteogenesis, adipogenesis, chondrogenesis and myogenesis. We also provide their respective histological analyses.

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Acknowledgements

This work was supported by the Slovenian Research Agency, J3-7245 Research Project and P3-0298 Research Programme.

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Authors and Affiliations

  1. Faculty of Pharmacy, Department of Clinical Biochemistry, University of Ljubljana, Ljubljana, Slovenia

    Klemen Čamernik & Janja Zupan

Authors
  1. Klemen Čamernik
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  2. Janja Zupan
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Corresponding author

Correspondence to Janja Zupan .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Čamernik, K., Zupan, J. (2018). Complete Assessment of Multilineage Differentiation Potential of Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cells. In: Turksen, K. (eds) Stem Cells and Aging . Methods in Molecular Biology, vol 2045. Humana, New York, NY. https://doi.org/10.1007/7651_2018_200

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  • DOI: https://doi.org/10.1007/7651_2018_200

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9712-1

  • Online ISBN: 978-1-4939-9713-8

  • eBook Packages: Springer Protocols

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