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Main Features of DNA-Based Vectors for Use in Lactic Acid Bacteria and Update Protocols

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Book cover DNA Vaccines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2197))

Abstract

DNA vaccines have been used as a promising strategy for delivery of immunogenic and immunomodulatory molecules into the host cells. Although, there are some obstacles involving the capability of the plasmid vector to reach the cell nucleus in great number to promote the expected benefits. In order to improve the delivery and, consequently, increase the expression levels of the target proteins carried by DNA vaccines, alternative methodologies have been explored, including the use of non-pathogenic bacteria as delivery vectors to carry, deliver, and protect the DNA from degradation, enhancing plasmid expression.

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Author information

Authors and Affiliations

  1. Laboratory of Cellular and Molecular Genetics, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Nina D. Coelho-Rocha, Fernanda A. L. Barroso, Laísa M. Tavares, Ester S. S. dos Santos, Vasco Azevedo, Mariana M. Drumond & Pamela Mancha-Agresti

  2. Center of Federal Education of Minas Gerais (CEFET-MG), Belo Horizonte, Minas Gerais, Brazil

    Mariana M. Drumond

Authors
  1. Nina D. Coelho-Rocha
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  2. Fernanda A. L. Barroso
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  3. Laísa M. Tavares
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  4. Ester S. S. dos Santos
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  5. Vasco Azevedo
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  6. Mariana M. Drumond
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  7. Pamela Mancha-Agresti
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Corresponding author

Correspondence to Pamela Mancha-Agresti .

Editor information

Editors and Affiliations

  1. CICS-UBI – Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal

    Ângela Sousa

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Coelho-Rocha, N.D. et al. (2021). Main Features of DNA-Based Vectors for Use in Lactic Acid Bacteria and Update Protocols. In: Sousa, Â. (eds) DNA Vaccines. Methods in Molecular Biology, vol 2197. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0872-2_16

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  • DOI: https://doi.org/10.1007/978-1-0716-0872-2_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0871-5

  • Online ISBN: 978-1-0716-0872-2

  • eBook Packages: Springer Protocols

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