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Impact of a Plasmid DNA-Based Alphavirus Vaccine on Immunization Efficiency

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DNA Vaccines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2197))

Abstract

Alphavirus vectors have been engineered for high-level gene expression relying originally on replication-deficient recombinant particles, more recently designed for plasmid DNA-based administration. As alphavirus-based DNA vectors encode the alphavirus RNA replicon genes, enhanced transgene expression in comparison to conventional DNA plasmids is achieved. Immunization studies with alphavirus-based DNA plasmids have elicited specific antibody production, have generated tumor regression and protection against challenges with infectious agents and tumor cells in various animal models. A limited number of clinical trials have been conducted with alphavirus DNA vectors. Compared to conventional plasmid DNA-based immunization, alphavirus DNA vectors required 1000-fold less DNA to elicit similar immune responses in rodents.

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Authors and Affiliations

  1. PanTherapeutics, Lutry, Switzerland

    Kenneth Lundstrom

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  1. Kenneth Lundstrom
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Correspondence to Kenneth Lundstrom .

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Editors and Affiliations

  1. CICS-UBI – Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal

    Ângela Sousa

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Lundstrom, K. (2021). Impact of a Plasmid DNA-Based Alphavirus Vaccine on Immunization Efficiency. In: Sousa, Â. (eds) DNA Vaccines. Methods in Molecular Biology, vol 2197. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0872-2_3

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  • DOI: https://doi.org/10.1007/978-1-0716-0872-2_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0871-5

  • Online ISBN: 978-1-0716-0872-2

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