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Assessing Antigen-Specific Cellular Immune Responses upon HIV /SIV Plasmid DNA Vaccination in the Nonhuman Primate Model

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Book cover DNA Vaccines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2197))

Abstract

Reliable detection and quantification of antigen-specific T cells are critical for assessing the immunogenicity of vaccine candidates. In this chapter, we describe the use of ELISpot and flow cytometry-based assays for efficient detection, mapping, and functional characterization of memory T lymphocytes in different tissues of rhesus macaques immunized with plasmid DNA. Flow cytometric assays provide a large amount of information, both phenotypic and functional, about individual cells, while the ELISpot is well suited for high throughput sample screening.

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Acknowledgement

This work was funded by the Intramural Research Program of the National Cancer Institute to B.K.F.. The contents of this chapter does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US government. We thank T. Jones for editorial assistance.

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Authors and Affiliations

  1. Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD, USA

    Xintao Hu & Barbara K. Felber

  2. Human Retrovirus Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD, USA

    Antonio Valentin

Authors
  1. Xintao Hu
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  2. Barbara K. Felber
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  3. Antonio Valentin
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Corresponding author

Correspondence to Xintao Hu .

Editor information

Editors and Affiliations

  1. CICS-UBI – Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal

    Ângela Sousa

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Hu, X., Felber, B.K., Valentin, A. (2021). Assessing Antigen-Specific Cellular Immune Responses upon HIV /SIV Plasmid DNA Vaccination in the Nonhuman Primate Model. In: Sousa, Â. (eds) DNA Vaccines. Methods in Molecular Biology, vol 2197. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0872-2_6

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  • DOI: https://doi.org/10.1007/978-1-0716-0872-2_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0871-5

  • Online ISBN: 978-1-0716-0872-2

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