Abstract
Alternative splicing allows genes to express isoforms with different coding or regulatory functions on demand. While short read deep sequencing technologies (RNA-seq) provide an immediate measurement of local splicing events, the phasing of these events along full-length isoforms requires the computational inference of long-range dependencies from short-range data points. We introduce CIDANE, a tool for the assembly and quantification of full-length isoforms from short read RNA-seq data. CIDANE bridges the gap between RNA quantification methods that rely on a complete annotation of a species’ transcriptome, and transcript assembly methods that will detect novel isoforms at the cost of a lower accuracy.
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Andreotti, S., Canzar, S. (2019). Guided Reconstruction of Full-Length Isoforms from Short Reads by CIDANE. In: Wajapeyee, N., Gupta, R. (eds) Epitranscriptomics. Methods in Molecular Biology, vol 1870. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8808-2_15
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DOI: https://doi.org/10.1007/978-1-4939-8808-2_15
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