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Profiling of N6-Methyladenosine in Zika Virus RNA and Host Cellular mRNA

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Epitranscriptomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1870))

Abstract

N6-Methylation of adenosine (m6A) in viral RNA was first identified in Rous sarcoma virus, influenza virus, and SV40 virus several decades ago. More recently, human immunodeficiency virus-1, hepatitis C virus, and Zika virus (ZIKV) RNAs have also been reported to be modified by m6A, highlighting the significance of this posttranscriptional RNA modification. Nevertheless, the functions of m6A and their roles in the context of viral infections are not completely understood. Similarly, the mechanisms by which viral infections may alter the dynamics of the host RNA methylome have yet to be elucidated. Here, we describe an experimental protocol to obtain m6A profiles of ZIKV RNA and the host cell mRNA using methylated RNA immunoprecipitation–sequencing (MeRIP-Seq).

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Authors and Affiliations

  1. Department of Pediatrics and Institute for Genomic Medicine, University of California San Diego, La Jolla, CA, USA

    Gianluigi Lichinchi & Tariq M. Rana

Authors
  1. Gianluigi Lichinchi
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  2. Tariq M. Rana
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Correspondence to Tariq M. Rana .

Editor information

Editors and Affiliations

  1. Department of Pathology, Yale University School of Medicine, New Haven, CT, USA

    Narendra Wajapeyee

  2. Yale Cancer Center, Yale University School of Medicine, New Haven, CT, USA

    Romi Gupta

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Lichinchi, G., Rana, T.M. (2019). Profiling of N6-Methyladenosine in Zika Virus RNA and Host Cellular mRNA. In: Wajapeyee, N., Gupta, R. (eds) Epitranscriptomics. Methods in Molecular Biology, vol 1870. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8808-2_16

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  • DOI: https://doi.org/10.1007/978-1-4939-8808-2_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8807-5

  • Online ISBN: 978-1-4939-8808-2

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