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Transgene Recombineering in Bacterial Artificial Chromosomes

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Microinjection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1874))

Abstract

Bacterial Artificial Chromosome (BAC) libraries are a valuable research resource. Any one of the clones in these libraries can carry hundreds of thousands of base pairs of genetic information. Often the entire coding sequence and significant upstream and downstream regions, including regulatory elements, can be found in a single BAC clone. BACs can be put to many uses, such as to study the function of human genes in knockout mice, to drive reporter gene expression in transgenic animals, and for gene discovery. In order to use BACs for experimental purposes it is often desirable to genetically modify them by introducing reporter elements or heterologous cDNA sequences. It is not feasible to use conventional DNA cloning approaches to modify BACs due to their size and complexity, thus a specialized field “recombineering” has developed to modify BAC clones through the use of homologous recombination in bacteria with short homology regions. Genetically engineered BACs can then be used in cell culture, mouse, or rat models to study cancer, neurology, and genetics.

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Author information

Authors and Affiliations

  1. University of Michigan Transgenic Animal Model Core, Ann Arbor, MI, USA

    Michael G. Zeidler & Thomas L. Saunders

  2. Division of Genetic Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA

    Thomas L. Saunders

Authors
  1. Michael G. Zeidler
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  2. Thomas L. Saunders
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Corresponding author

Correspondence to Thomas L. Saunders .

Editor information

Editors and Affiliations

  1. National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA

    Chengyu Liu

  2. National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA

    Yubin Du

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Zeidler, M.G., Saunders, T.L. (2019). Transgene Recombineering in Bacterial Artificial Chromosomes. In: Liu, C., Du, Y. (eds) Microinjection. Methods in Molecular Biology, vol 1874. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8831-0_3

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  • DOI: https://doi.org/10.1007/978-1-4939-8831-0_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8830-3

  • Online ISBN: 978-1-4939-8831-0

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