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Hydrogenases

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Metalloproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1876))

Abstract

Hydrogenases catalyze the simple yet important interconversion between H2 and protons and electrons. Found throughout prokaryotes, lower eukaryotes, and archaea, hydrogenases are used for a variety of redox and signaling purposes and are found in many different forms. This diverse group of metalloenzymes is divided into [NiFe], [FeFe], and [Fe] variants, based on the transition metal contents of their active sites. A wide array of biochemical and spectroscopic methods has been used to elucidate hydrogenases, and this along with a general description of the main enzyme types and catalytic mechanisms is discussed in this chapter.

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Acknowledgments

The authors are supported by the National Institutes of Health grant GM67626 (to M.W.R. and Y.H.).

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  1. Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USA

    Nathaniel S. Sickerman & Yilin Hu

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  1. Nathaniel S. Sickerman
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Correspondence to Yilin Hu .

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  1. Department of Molecular Biology and Biochemistry, University of California-Irvine, Irvine, CA, USA

    Yilin Hu

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Sickerman, N.S., Hu, Y. (2019). Hydrogenases. In: Hu, Y. (eds) Metalloproteins. Methods in Molecular Biology, vol 1876. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8864-8_5

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  • DOI: https://doi.org/10.1007/978-1-4939-8864-8_5

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