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Characterization of FOXO Acetylation

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FOXO Transcription Factors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1890))

Abstract

FOXO3 is a tumor suppressor that orchestrates the expression of genes that regulate cell cycle progression, apoptosis, metabolism, oxidative stress, and other important cellular processes. Its inactivation is closely associated with tumorigenesis and cancer progression. On the other hand, sirtuin proteins have been demonstrated to be able to deacetylate, thus causing FOXO3 inactivation at the posttranslational level. Therefore, targeting sirtuin proteins renders new avenues for breast cancer treatment. Here, we describe three procedures for studying FOXO3 posttranslational modifications controlled by sirtuin proteins in cancer cells.

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Author information

Authors and Affiliations

  1. Department of Surgery and Cancer, Imperial College London, Imperial Centre for Translational and Experimental Medicine (ICTEM), London, UK

    Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-García & Eric W-F Lam

  2. Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology - Hellas (FORTH), Heraklion, Crete, Greece

    Nikoleta Sachini

  3. Department of Biology, University of Crete, Heraklion, Crete, Greece

    Nikoleta Sachini

  4. Multidisciplinary Unit, Faculty of Science, Mahidol University, Bangkok, Thailand

    Sathid Aimjongjun

Authors
  1. Shang Yao
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  2. Zimam Mahmud
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  3. Nikoleta Sachini
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  4. Sathid Aimjongjun
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  5. Paula Saavedra-García
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  6. Eric W-F Lam
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Corresponding author

Correspondence to Eric W-F Lam .

Editor information

Editors and Affiliations

  1. Instituto de Investigaciones Biomédicas “Alberto Sols” (CSIC-UAM), Madrid, Spain

    Wolfgang Link

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Yao, S., Mahmud, Z., Sachini, N., Aimjongjun, S., Saavedra-García, P., Lam, E.WF. (2019). Characterization of FOXO Acetylation. In: Link, W. (eds) FOXO Transcription Factors. Methods in Molecular Biology, vol 1890. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8900-3_7

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  • DOI: https://doi.org/10.1007/978-1-4939-8900-3_7

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-8899-0

  • Online ISBN: 978-1-4939-8900-3

  • eBook Packages: Springer Protocols

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