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Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation

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Neural Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1919))

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Abstract

Lentiviral vectors are increasingly used as efficient gene transfer tools in the experimental and clinical gene therapy treatment of acquired and inherited genetic diseases. Hematopoietic stem cells (HSCs) are characterized by the capacity for self-renewal, as well as multi-lineage differentiation and maintenance of the lymphohematopoietic system throughout life. As such, HSC transplantation (HSCT) has proven to be a powerful therapeutic modality for the treatment of both malignant and nonmalignant disorders. Transduction of lentiviral vectors into HSCs may offer long-term stable expression of a therapeutic gene in both preclinical and clinical settings. The purpose of this chapter is to describe an optimized procedure for lentiviral transduction of mouse HSCs followed by HSCT.

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Acknowledgment

The establishment of this method was supported by a Merit Review grant (2I01BX000737) from the Department of Veterans Affairs Biomedical Laboratory Research & Development Program and by the William and Ella Owens Medical Research Foundation.

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Authors and Affiliations

  1. Department of Medicine, UT Health Science Center at San Antonio, San Antonio, TX, USA

    Cang Chen, Michael J. Guderyon, Guo Ge, Robert A. Clark & Senlin Li

  2. Research and Development Service, Audie L. Murphy VA Hospital, San Antonio, TX, USA

    Robert A. Clark & Senlin Li

  3. Department of Pharmacology, University of Texas Health San Antonio, San Antonio, TX, USA

    Senlin Li

Authors
  1. Cang Chen
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  2. Michael J. Guderyon
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  3. Guo Ge
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  4. Robert A. Clark
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  5. Senlin Li
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Corresponding author

Correspondence to Senlin Li .

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Editors and Affiliations

  1. Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA

    Marcel M. Daadi

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Chen, C., Guderyon, M.J., Ge, G., Clark, R.A., Li, S. (2019). Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation. In: Daadi, M. (eds) Neural Stem Cells. Methods in Molecular Biology, vol 1919. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9007-8_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9007-8_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9005-4

  • Online ISBN: 978-1-4939-9007-8

  • eBook Packages: Springer Protocols

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