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Development of Taxus spp. Hairy Root Cultures for Enhanced Taxane Production

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Plant Cell and Tissue Differentiation and Secondary Metabolites

Part of the book series: Reference Series in Phytochemistry ((RSP))

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Abstract

The history of development of Taxus spp. hairy root cultures for taxane production is reviewed. The multiple efforts leading to establishing yew hairy root cultures are presented. Moreover various biotechnological approaches elaborated to enhance taxane production are discussed. They include precursor and elicitor feeding, two-phase cultures, as well as metabolic engineering approach.

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Abbreviations

½ MS:

Half strength Murashige and Skoog (1962) medium [42]

½ LM:

Half strength Litvay et al. (1981) medium [40]

10 DAB III:

10-Deacetylbaccatin III

B5:

Gamborg et al. (1968) medium [41]

BAP:

6-Benzylaminopurine

BAPT :

Baccatin III: 3-amino, 3-phenylpropanoyltransferase

DBTNBT :

3′-N-Debenzoyl-2-deoxytaxol-N-benzoyltransferase

DCR:

Gupta and Durzan (1985) medium [48]

DCR-M:

Gupta et Durzan (1985) medium modified by augmentation of MgSO4 × 7H2O up to 400 mg L−1 and addition of L-glutamine 500 mg L−1 [50]

DW:

Dry weight

FW:

Fresh weight

IBA:

Indole-3-butyric acid

MeJa:

Methyl jasmonate

MPM:

Mycelia of the pine mushroom

MS:

Murashige et Skoog (1962) medium [42]

NAA:

1-Naphthaleneacetic acid

PABA:

p-Amino benzoic acid

PAL:

L-Phenylalanine ammonia lyase

PCR:

Polymerase chain reaction

PFD:

Perfluorodecalin

PHEN:

L-Phenylalanine

SA:

Salicylic acid

SH:

Schenk and Hildebrandt medium (1972) [54]

SNP:

Sodium nitroprusside

TXS :

Taxadiene synthase

WPM:

Woody plant medium by McCown and Lloyd (1981) [53]

YEB:

Medium for bacteria cultivation by Van Larebeke et al. [57]

YEP:

Yeast extract peptone medium for bacterial cultures [52]

YMB:

Yeast mannitol broth medium for bacterial cultures [49]

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Author information

Authors and Affiliations

  1. Department of Pharmaceutical Biology and Medicinal Plant Biotechnology, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland

    Katarzyna Sykłowska-Baranek & Agnieszka Pietrosiuk

  2. Department of Clinical Chemistry and Laboratory Diagnostics, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland

    Grażyna Sygitowicz

Authors
  1. Katarzyna Sykłowska-Baranek
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  2. Grażyna Sygitowicz
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  3. Agnieszka Pietrosiuk
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Corresponding author

Correspondence to Katarzyna Sykłowska-Baranek .

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Editors and Affiliations

  1. Department of Botany, M.L.Sukhadia university, Udaipur, Rajasthan, India

    Kishan Gopal Ramawat

  2. Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Kraków, Poland

    Halina Maria Ekiert

  3. Amarillo, TX, USA

    Shaily Goyal

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Sykłowska-Baranek, K., Sygitowicz, G., Pietrosiuk, A. (2019). Development of Taxus spp. Hairy Root Cultures for Enhanced Taxane Production. In: Ramawat, K., Ekiert, H., Goyal, S. (eds) Plant Cell and Tissue Differentiation and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-11253-0_22-1

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  • DOI: https://doi.org/10.1007/978-3-030-11253-0_22-1

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  • Print ISBN: 978-3-030-11253-0

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