Whole Genome Amplification

Introduction

DNA samples obtained from different sources, namely, single cell (Zhang et al. 1992), tissue biopsies, culture cells, and forensic and tumor samples, may be limited in quantity for various analysis. Amplification of DNA fragments for further experiments can be obtained through different types of polymerase chain reaction (PCR). These techniques have some challenges such as limited DNA yield, and only a particular region of chromosome can be studied. To study the whole genome, the demands cannot be met by using these techniques. These limitations paved a way to develop a new technique to overcome these problems and get a good yield of DNA.

What Is WGA?

Whole genome amplification (WGA) technique involves modification of conventional PCR method through which the whole genome gets covered to give a good amplification efficiency. Micrograms of DNA can be synthesized from ng or lesser amounts.

PCR-based WGA includes:

• 1. LA-PCR (linker adapter/ligation-anchored PCR)

• 2. IRS PCR...