Introduction
Signaling cascades involving cyclic nucleotides play key roles in signal transduction in virtually all cell types. A current challenge is to decipher the spatiotemporal regulation of cyclic nucleotides pathways that any given cell type has concocted to be able to discriminate among different external stimuli. For more than 20 years, information on cyclic nucleotide signaling in cardiac preparations were gathered using conventional biochemical assays in fractionated dead tissues or cells. However, real time measurement of localized cyclic adenosine 3′, 5′-monophosphate (cAMP) and cyclic guanosine 3′, 5′-monophosphate (cGMP) signals as evidence supporting a compartmentation of these cyclic nucleotides was difficult to achieve using these methods in living cells. Since then, the advent of live cell imaging techniques allowed a new visualization and understanding of the spatiotemporal organization of the cyclic nucleotides signals at the cellular, subcellular, and tissue...
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Abi-Gerges, A., Khalil, K.N., Neaimeh, Y.R., Fischmeister, R. (2019). Real-Time Monitoring of Cyclic Nucleotide Changes in Living Cells. In: Roberts, G., Watts, A. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35943-9_377-1
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