Definitions
Electron crystallography of 2D crystals: A procedure where electron diffraction data and electron microscope images taken from 2D crystals are used to determine the structure of biological macromolecules.
Introduction
Electron crystallography of 2D crystals is a method to determine protein structure at high resolution using electron microscope. This technique was originally developed by Henderson and Unwin in their structural studies of purple membrane (Henderson and Unwin 1975). They obtained the density map of bacteriorhodopsin (bR) at 7-Å resolution, showing the first structure of a membrane protein which consists of seven membrane-spanning α-helices. Henderson and co-workers improved their specimen preparation and data processing methods and finally presented the first atomic model of bR by electron crystallography (Henderson et al. 1990). Initially, this method was applied for...
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References
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Murata, K. (2020). Electron Crystallography of 2D Crystals. In: Roberts, G., Watts, A. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35943-9_621-1
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DOI: https://doi.org/10.1007/978-3-642-35943-9_621-1
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