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Amperometric Biosensors

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  • First Online:
Encyclopedia of Biophysics

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Synonyms

Enzyme electrodes

Definition

Amperometric biosensors are self-contained integrated devices based on the measurement of the current resulting from the oxidation or reduction of an electroactive biological element providing specific quantitative analytical information.

Introduction

Determining analyte concentrations is essential for a wide range of applications, including high-throughput drug screening, disease diagnosis and treatment, quality control, and environmental monitoring. The research in the field of biosensors, and amperometric biosensors in particular, was initiated by Clark whose study on the oxygen electrode was published in 1956 (Clark 1956). Since then electrochemical biosensors have been widely used to determine the analyte concentrations, both in research and commercial applications.

A biosensor comprises of two closely associated elements: a biological recognition element and a signal conversion unit, the physicochemical transducer, which eventually produce a...

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References

  • Bartlett PN (2008) Bioelectrochemistry fundamentals, experimental techniques and applications. In: Bartlett PN (ed). Wiley, West Sussex

    Google Scholar 

  • Belluzo MS, Ribone ME et al (2008) Assembling Amperometric biosensors for clinical diagnostics. Sensors 8:1366–1399

    Article  CAS  Google Scholar 

  • Cass AEG, Davis G et al (1984) Ferrocene-mediated enzyme electrode for amperometric determination of glucose. Anal Chem 56:667–671

    Article  CAS  Google Scholar 

  • Chaki NK, Vijayamohanan K (2002) Self-assembled monolayers as a tunable platform for biosensor applications. Biosens Bioelectron 17:1–12

    Article  CAS  Google Scholar 

  • Chaubey A, Malhotra BD (2002) Mediated biosensors. Biosens Bioelectron 17:441–456

    Article  CAS  Google Scholar 

  • Clark LC (1956) Monitor and control of blood and tissue oxygen tensions. Trans Am Soc Artif Intern Organs 2:41–48

    Google Scholar 

  • Eggins BR (2002) Chemical sensors and biosensors. Wiley, West Sussex

    Google Scholar 

  • Emr SA, Yacynych AM (1995) Use of polymer films in amperometric biosensors. Electroanalysis 7:913–923

    Article  CAS  Google Scholar 

  • Ferrero VE, Andolfi L et al (2008) Protein and electrode engineering for the covalent immobilisation of P450 BMP on gold. Anal Chem 80:8438–8446

    Article  CAS  Google Scholar 

  • Haensch C, Hoeppener S et al (2010) Chemical modification of self-assembled silane based monolayers by surface reactions. Chem Soc Rev 39:2323–2334

    Article  CAS  Google Scholar 

  • Hill HAO, Sanghera GS (1990) Mediated amperometric enzyme electrodes. In: Cass AEG (ed) Biosensors-A practical approach. IRL Press, Oxford, p 19

    Google Scholar 

  • Holzinger M, Le Goff A et al (2014) Nanomaterials for biosensing applications: a review. Front Chem 2:63

    Article  Google Scholar 

  • Krishnan SK, Singh E et al (2019) A review on graphene-based nanocomposites for electrochemical and fluorescent biosensors. RSC Adv 9:8778–8881

    Article  CAS  Google Scholar 

  • Mak LH, Sadeghi SJ et al (2010) Control of human cytochrome P450 2E1 electrocatalytic response as a result of unique orientation on gold electrodes. Anal Chem 82:5357–5362

    Article  CAS  Google Scholar 

  • Marcus RA, Sutin N (1985) Electron transfer in chemistry and biology. Biochim Biophys Acta 811:265–322

    Article  CAS  Google Scholar 

  • Pratibha P, Monika D et al (2008) Prospects of nanomaterials in biosensors. Anal Lett 41:159–209

    Article  Google Scholar 

  • Rivas GA, Rubianes MD et al (2007) Carbon nanotubes for electrochemical biosensing. Talanta 74:291–307

    Article  CAS  Google Scholar 

  • Ronkainen NJ, Halsall HB et al (2010) Electrochemical biosensors. Chem Soc Rev 39:1747–1763

    Article  CAS  Google Scholar 

  • Sadeghi SJ, Gilardi G et al (1997) Mediated electrochemistry of peroxidases – effects of variations in protein and mediator structures. Biosens Bioelectron 12:1191–1198

    Article  CAS  Google Scholar 

  • Sadeghi SJ, Meirinhos R et al (2010) Direct electrochemistry of drug metabolizing human flavin-containing monooxygenase: electrochemical turnover of benzydamine and tamoxifen. J Am Chem Soc 132:458–459

    Article  CAS  Google Scholar 

  • Sadeghi SJ, Fantuzzi A et al (2011) Breakthrough in P450 bioelectrochemistry and future perspectives. BBA – Proteins Proteomics 1814:237–248

    Article  CAS  Google Scholar 

  • Samanta D, Sarkar A (2011) Immobilization of bio-macromolecules on self-assembled monolayers: methods and sensor applications. Chem Soc Rev 40:2567–2592

    Article  Google Scholar 

  • Su H, Li S et al (2017) Nanomaterial-based biosensors for biological detections. Adv Health Care Tech 3:19–29

    Article  Google Scholar 

  • Wightman RM, Wipf DO (1989) Electroanalytical chemistry. In: Bard AJ (ed). Marcel Dekker, New York, p 267

    Google Scholar 

  • Yang C, Huang U et al (2009) Amperometric electrochemical microsystem for a miniaturized protein biosensor array. IEEE Trans Biomed Circuits Syst 3:160–168

    Article  Google Scholar 

  • Yuan M, Minteer SD (2019) Redox polymers in electrochemical systems: from methods to mediation to energy storage. Curr Opin Electrochem 15:1–6

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Life Sciences and Systems Biology, University of Torino, Turin, Italy

    Sheila J. Sadeghi

Authors
  1. Sheila J. Sadeghi
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Corresponding author

Correspondence to Sheila J. Sadeghi .

Editor information

Editors and Affiliations

  1. University Leicester MRC Centre, Leicester, UK

    Gordon Roberts

  2. Dept Biochemistry, University of Oxford, Oxford, UK

    Anthony Watts

Section Editor information

  1. No affiliation provided

    Tony Cass

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© 2021 European Biophysical Societies' Association (EBSA)

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Sadeghi, S.J. (2021). Amperometric Biosensors. In: Roberts, G., Watts, A. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35943-9_713-2

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  • DOI: https://doi.org/10.1007/978-3-642-35943-9_713-2

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-35943-9

  • Online ISBN: 978-3-642-35943-9

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

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