Abstract
Sargassum are brown algae belonging to the class Phaeophyceae. Brown algae are rich in nutrients and widely used in food. Most previous experiments have focused on the functional evaluation of organic solvent extracts of Sargassum. Considering food safety, this study investigated the antioxidant and antiobesity activities of Sargassum hemiphyllum water extract (SE). The antioxidant activity of SE (500–4000 mg/mL) was determined in vitro. The results indicated that SE has good DPPH radical scavenging activity (14–74%), reducing power (20–78%), ABTS+ radical scavenging activity (8–91%), and Fe2+ chelating ability (5–25%). Furthermore, the antiobesity activity of SE (50–300 mg/mL) was analysed in a 3T3-L1 adipocyte model. SE effectively inhibited lipid accumulation (determined by methods including measuring the absorbance of Oil red O after staining and the triglyceride content, which were decreased by 10% and 20%, respectively) by reducing peroxisome proliferator-activated receptor gamma (PPARγ) protein expression in 3T3-L1 adipocytes. This study suggested that SE has good antioxidant and antiobesity properties.
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The datasets generated and/or analyzed during the current study are not publicly available due [REASON WHY DATA ARE NOT PUBLIC] but are available from the corresponding author at reasonable request.
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Abbreviations
- SE:
-
Sargassum hemiphyllum Water extract
- WHO:
-
World Health Organization
- PPARγ:
-
Peroxisome proliferator-activated receptor gamma
- C/EBPα:
-
CCAT/enhancer-binding protein alpha
- SREBP-1c:
-
Sterol regulatory element-binding protein-1c
- PBS:
-
Phosphate-buffered saline
- DPPH:
-
α, α-Diphenyl-β-picrylhydrazyl
- ABTS:
-
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
- Dex:
-
Dexamethasone
- MTT:
-
3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
- IBMX:
-
3-Isobutyl-methylxanthine
- FBS:
-
Fetal bovine serum
- TG:
-
Triglyceride
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Conceptualization, C-CW, J-JW and S-LH; Methodology, C-CW and J-JW; Validation, Y-TC and Y-WH; Formal analysis, Y-TC; Investigation, Y-WH, T-YS and Y-TC; Data curation, Y-WH and T-YS; Writing-original draft preparation, Y-TC and Y-WH; Writing-review and editing, J-JW; Supervision, S-LH; Project administration, S-LH. All authors read and agreed to the published version of the manuscript.
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Chen, YT., Huang, YW., Shen, TY. et al. Evaluation of antioxidant and anti-obesity potential of Sargassum extracts. J Food Sci Technol 60, 1723–1730 (2023). https://doi.org/10.1007/s13197-023-05707-1
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DOI: https://doi.org/10.1007/s13197-023-05707-1