Abstract
Identifying the risk of ochratoxin A in our daily food has become fundamental because of its toxicity. In this work, we report a novel semi-automated in-syringe-based fast mycotoxin extraction (IS-FaMEx) technique coupled with direct-injection electrospray-ionization tandem mass spectrometer (ESI-MS/MS) detection for the quantification of ochratoxin A in coffee and tea samples. Under the optimized conditions, the results reveal that the developed method’s linearity was more remarkable, with a correlation coefficient of > 0.999 and > 92% extraction recovery with a precision of 6%. The detection and quantification limits for ochratoxin A were 0.2 and 0.8 ng g−1 for the developed method, respectively, which is lower than the European Union regulatory limit of toxicity for ochratoxin-A (5 ng g−1) in coffee. Furthermore, the newly developed modified IS-FaMEx-ESI-MS/MS exhibited lower signal suppression of 8% with a good green metric score of 0.64. In addition, the IS-FaMEx-ESI-MS/MS showed good extraction recovery, matrix elimination, good detection, and quantification limits with high accuracy and precision due to the fewer extraction steps with semi-automation. Therefore, the presented method can be applied as a potential methodology for the detection of mycotoxins in food products for food safety and quality control purposes.
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The datasets used during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
Authors like to thank Kaohsiung Medical University for providing the vital resources for this research.
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This work was supported partially by the Research Center for Precision Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan and by Kaohsiung Medical University Research Center Grant (KMUTC112A01). This work was also supported financially by the grant MOST-110-2113-M-037-009- from the Ministry of Science and Technology, Taiwan. This research work was also supported financially by the bilateral grant from 109KN007 (KMU-NSYSU), Taiwan.
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KP, SG: Methodology, Investigation, Formal analysis, Validation, Writing-Original Draft; JS, MTU, RRS, AKP: Conceptualization, Resources, Investigation, Validation; CDD, YCL, BT, PCH, GA: Data Curation, Visualization; Supervision; VKP: Methodology, Resources, Writing—Review & Editing, Supervision, and Funding acquisition.
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Prakasham, K., Gurrani, S., Shiea, J. et al. Facile analysis of mycotoxin in coffee and tea samples using a novel semi-automated in-syringe based fast mycotoxin extraction (FaMEx) technique coupled with direct-injection ESI-MS/MS analysis. J Food Sci Technol 60, 1992–2000 (2023). https://doi.org/10.1007/s13197-023-05733-z
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DOI: https://doi.org/10.1007/s13197-023-05733-z