Abstract
Since many tumors are associated with a pronounced collagen-rich stromal reaction, there is increasing interest in understanding mechanisms by which cancer cells invade through the collagen barrier. Here we describe a quantitative method to track cell invasion in 3D collagen I gels. We analyze invasion by quantifying proteolytic tracks generated by invading cancer cells through a 3D collagen microenvironment. We provide a detailed protocol for this quantitative assay, which can be used to characterize signaling pathways that regulate invasion in the 3D microenvironment.
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Acknowledgments
This work was supported by R01CA186885 grant (to H.G. Munshi) from the NCI and a Merit award I01BX002922 (to H.G. Munshi) from the Department of Veterans Affairs.
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Ebine, K., Chow, C.R., Munshi, H.G. (2019). Quantitative Method to Track Proteolytic Invasion in 3D Collagen. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 1882. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8879-2_15
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DOI: https://doi.org/10.1007/978-1-4939-8879-2_15
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