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Methods for Monitoring Macroautophagy in Pancreatic Cancer Cells

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Pancreatic Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1882))

Abstract

Macroautophagy is a catabolic process through which redundant, aged, or damaged cellular structures are first enclosed within double-membrane vesicles (called autophagosomes), and thereafter degraded within lysosomes. Macroautophagy provides a primary route for the turnover of macromolecules, membranes and organelles, and as such plays a major role in cell homeostasis. As part of the stress response, autophagy is crucial to determine the cell fate in response to extracellular or intracellular injuries. Autophagy is involved in cancerogenesis and in cancer progression. Here we illustrate the essential methods for monitoring autophagy in pancreatic cancer cells.

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Pathology and Nanobioimaging, Department of Health Sciences, Università del Piemonte Orientale, Novara, Italy

    Chiara Vidoni, Alessandra Ferraresi, Christian Seca, Eleonora Secomandi & Ciro Isidoro

Authors
  1. Chiara Vidoni
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  2. Alessandra Ferraresi
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  3. Christian Seca
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  4. Eleonora Secomandi
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  5. Ciro Isidoro
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Corresponding author

Correspondence to Ciro Isidoro .

Editor information

Editors and Affiliations

  1. Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA

    Gloria H. Su

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Vidoni, C., Ferraresi, A., Seca, C., Secomandi, E., Isidoro, C. (2019). Methods for Monitoring Macroautophagy in Pancreatic Cancer Cells. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 1882. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8879-2_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8879-2_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8878-5

  • Online ISBN: 978-1-4939-8879-2

  • eBook Packages: Springer Protocols

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