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Induction of Pancreatic Inflammation Accelerates Pancreatic Tumorigenesis in Mice

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Pancreatic Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1882))

Abstract

Pancreatitis is a major risk factor for the development of pancreatic cancer. In genetically engineered mouse models, induction of pancreatic inflammation dramatically accelerates oncogenic KRas-induced fibrosis, precancerous PanIN formation, and tumorigenesis. Here we describe simple methods of secretagogue-induced experimental acute and chronic pancreatitis, the most commonly used pancreatitis models, and their applications in pancreatic cancer research. Additionally, the preparation of primary pancreatic acinar cells is introduced. Primary acinar cells can be used to study the early events of pancreatic inflammation and pancreatic acinar-to-ductal (ADM) metaplasia.

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Acknowledgment

The work presented in this chapter was supported by Grant W81XWH-15-1-0257 from the US Department of Defense. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Department of Defense.

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Authors and Affiliations

  1. Department of Cancer Biology, Mayo Clinic, Jacksonville, FL, USA

    Lu Zhuang & Baoan Ji

  2. Department of Oncology, Changhai Hospital, Shanghai, China

    Xianbao Zhan

  3. Department of Gastroenterology, Mayo Clinic, Jacksonville, FL, USA

    Yan Bi

Authors
  1. Lu Zhuang
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  2. Xianbao Zhan
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  3. Yan Bi
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  4. Baoan Ji
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Corresponding author

Correspondence to Baoan Ji .

Editor information

Editors and Affiliations

  1. Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA

    Gloria H. Su

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Zhuang, L., Zhan, X., Bi, Y., Ji, B. (2019). Induction of Pancreatic Inflammation Accelerates Pancreatic Tumorigenesis in Mice. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 1882. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8879-2_25

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  • DOI: https://doi.org/10.1007/978-1-4939-8879-2_25

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8878-5

  • Online ISBN: 978-1-4939-8879-2

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