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An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility

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T-Cell Motility

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1930))

Abstract

The inherent ability of T-cells to migrate is critical for a fully functional immune system, both in normal immune surveillance and for mounting an adaptive immune response. At the same time, inappropriate trafficking of T-cells can be a pathological factor for immune-mediated or chronic inflammatory diseases. T-cell motility is critically dependent on a series of ligand–receptor interactions, a precisely regulated intracellular signaling, an involvement of adaptor proteins, and dynamic remodeling of the cytoskeletal systems. The leukocyte integrin LFA-1 receptor present on T-cells binds to the ligand intercellular adhesion molecule 1 (ICAM-1) and this LFA-1/ICAM-1 contact acts as a trigger for T-cell motility. In this book, we present a collection of methods and protocols that are frequently used by researchers to better understand T-cell motility in health and diseases.

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Acknowledgments

This work was supported by the Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Start-Up Grant and the Singapore Ministry of Education (MOE) under its Singapore MOE Academic Research Fund (AcRF) Tier 2 Grant (MOE2017-T2-2-004) to N.K.V.

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Authors and Affiliations

  1. Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore

    Navin Kumar Verma

  2. Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore

    Dermot Kelleher

  3. Departments of Medicine and Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada

    Dermot Kelleher

Authors
  1. Navin Kumar Verma
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  2. Dermot Kelleher
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Corresponding author

Correspondence to Navin Kumar Verma .

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Editors and Affiliations

  1. Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore

    Navin Kumar Verma

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Verma, N.K., Kelleher, D. (2019). An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility. In: Verma, N. (eds) T-Cell Motility. Methods in Molecular Biology, vol 1930. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9036-8_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9036-8_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9035-1

  • Online ISBN: 978-1-4939-9036-8

  • eBook Packages: Springer Protocols

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