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Different Types of In Vitro Cultures of Schisandra chinensis and Its Cultivar (S. chinensis cv. Sadova): A Rich Potential Source of Specific Lignans and Phenolic Compounds

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Plant Cell and Tissue Differentiation and Secondary Metabolites

Part of the book series: Reference Series in Phytochemistry ((RSP))

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Abstract

Chinese magnolia vine (Schisandra chinensis) is a well-known traditional Chinese medicinal plant species, which is very important in modern phytotherapy. The key role is assigned to specific compounds – dibenzocyclooctadiene lignans. This chapter describes the use of S. chinensis in vitro cultures as a tool to increase their production under controlled conditions as a promising biotechnological alternative to their extraction from ex vitro plant material or by chemical synthesis. Moreover, the chosen phenolic compounds (phenolic acids and flavonoids) were taken into consideration, too. The whole process of biotechnological research was applied to studying S. chinensis and its cultivar – S. chinensis cv. Sadova No. 1. The studies involved initiation of in vitro cultures, optimization of the basal composition of the culture media, duration of the growth cycles, type of cultures, culture lighting conditions, elicitors, and bioreactor design. In all the steps of biotechnological process, efficient production of specific lignans and phenolic compounds was achieved. The research proved that plant in vitro cultures of both Schisandras can be an efficient tool used to increase the production of the desired secondary metabolites.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

BA:

6-Benzyladenine

DW:

Dry weight

GA3:

Gibberellic acid

LC-DAD:

Liquid chromatography with diode-array detection

LC-DAD-ESI-MS:

Liquid chromatography with diode-array detection and electrospray ionization mass spectrometry

LC-UV:

Liquid chromatography with ultraviolet-visible detector

LS:

Linsmaier and Skoog

MS:

Murashige and Skoog

NAA:

1-Naphthaleneacetic acid

PGRs:

Plant growth regulators

WV5:

Westvaco

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Acknowledgments

This study was supported the financial support of the funds of the Ministry of Science and Higher Education Programs: K/DSC/000029, K/DSC/001950, K/DSC/004297, K/ZDS/003312, K/ZDS/005614, and National Science Centre, Poland (grant number 2016/23/D/NZ7/01316).

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Authors and Affiliations

  1. Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Kraków, Poland

    Agnieszka Szopa, Marta Klimek-Szczykutowicz & Halina Ekiert

  2. Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk, Gdańsk, Poland

    Adam Kokotkiewicz & Maria Luczkiewicz

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  1. Agnieszka Szopa
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  2. Adam Kokotkiewicz
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  5. Halina Ekiert
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Correspondence to Agnieszka Szopa .

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Editors and Affiliations

  1. Department of Botany, M.L.Sukhadia university, Udaipur, Rajasthan, India

    Kishan Gopal Ramawat

  2. Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Kraków, Poland

    Halina Maria Ekiert

  3. Amarillo, TX, USA

    Shaily Goyal

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Szopa, A., Kokotkiewicz, A., Klimek-Szczykutowicz, M., Luczkiewicz, M., Ekiert, H. (2020). Different Types of In Vitro Cultures of Schisandra chinensis and Its Cultivar (S. chinensis cv. Sadova): A Rich Potential Source of Specific Lignans and Phenolic Compounds. In: Ramawat, K., Ekiert, H., Goyal, S. (eds) Plant Cell and Tissue Differentiation and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-11253-0_10-2

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  1. Latest

    cv. Sadova): A Rich Potential Source of Specific Lignans and Phenolic Compounds
    Published:
    01 January 2020

    DOI: https://doi.org/10.1007/978-3-030-11253-0_10-2

  2. Original

    cv. Sadova): A Rich Potential Source of Specific Lignans and Phenolic Compounds
    Published:
    28 November 2019

    DOI: https://doi.org/10.1007/978-3-030-11253-0_10-1

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